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For the enumeration of cells in order to produce genomic DNA standards for real-time PCR quantification, DNA was extracted from an aliquot of the filtered culture with the DNeasy Blood & Tissue Kit (Qiagen Ltd., Crawley, UK).
The method of enumeration of cells in a VCC assay is confounded if the cells form clumps, because that clumping and biofilm formation affects optical density readings.
However, most of these markers are basal markers involving the enumeration of cells or factors in a sample without controlled experimental stimulation to assess a specific functional response.
Under controlled conditions of reproducibly extracted, good-quality RNA, initial gene transcript number is ideally standardised to cell number, but accurate enumeration of cells is often precluded when starting with solid tissue [ 49].
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Using commercial software for flow cytometric analysis, enumeration of cell division history can next be performed to model proliferation.
For the enumeration of cell-in-cell frequency per sample, cells from 40× magnification fields were counted and the cell-in-cell frequency was represented as mean ± SEM from at least three different fields.
The first measure is based on the simple enumeration of cell activities that, as an effect of uncoupling, resulted enriched uniquely in the transcriptome or in the translatome DEGs.
Enumeration of CD34+ cells in leukocyte-rich cell suspensions is important for clinical decision-making in stem cell transplantation.
For instance, the following sentence contains an enumeration of endocrine cells: '… several adenohypophysial endocrine cells such as somatotrophs, thyrotrophs, and gonadotrophs' [ http://www.ncbi.nlm.nih.gov/pubmed/11478270].nih.gov/pubmed/11478270]
The enumeration of blood cells was done in a Cell Dyn Sapphire® Analyzer (Abbot Diagnostics, Wavre, Belgium).
Enumeration of imaged cells revealed that > 99.0% of leachate-exposed cells remain intact and that damaged or lysed cells were only observed in < 1% of CB07-L and BY07-L-exposed cells.
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