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After enumerating with a blood cell counter, the bacterial cells were washed three times with sterile PBS, re-suspended at a density of 1.33×108 cells/ml, and used for the bacterial challenge experiments.
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The problems can be enumerated with a prim scowl and a wagging finger.
I sat with them on their mossy rear terrace while they enumerated, with a kind of mellow resignation, their house's problems.
The bacterial suspension in PBS was enumerated with a UV-visible spectrophotometer and inactivated with 1% (v/v) formalin for 1 h at 30°C.
Bead numbers were enumerated with a Reichert Bright-Line Metallized Hemacytometer from Hausser Scientific (Horsham, PA).
The yeasts were centrifuged and washed twice in sterile saline, resuspended in saline, and enumerated with a hemocytometer.
Samples were enumerated with a hemocytometer for total leukocyte number.
Colonies were enumerated with a laser colony counter (Spiral System) after 48 h of incubation at 37°C.
Third, the S1-S2 components were enumerated with a 3′-dT20 appendage.
Stationary-phase promastigotes were harvested by centrifugation, washed in NaCl 0.3%, and enumerated with a Thoma hemacytometer.
Total leukocytes in BALF were enumerated with a hemocytometer, and fractions of eosinophils, neutrophils, macrophages, and lymphocytes were determined in a cytospin sample stained with Diff-Quick.
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