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PBMC were enumerated with an automated Guava cell counter (Millipore, Billerica, MA).
The agar plates were incubated for 28 days at 37°C/5% CO2 and individual M. avium colonies were enumerated with an inverted microscope after 14 and 28 days of incubation.
Spots were enumerated with an AID ViruSpot plate reader and ViruSpot 3.3 software (Autoimmun Diagnostika GmbH, Germany).
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The problems can be enumerated with a prim scowl and a wagging finger.
I sat with them on their mossy rear terrace while they enumerated, with a kind of mellow resignation, their house's problems.
The bacterial suspension in PBS was enumerated with a UV-visible spectrophotometer and inactivated with 1% (v/v) formalin for 1 h at 30°C.
Bead numbers were enumerated with a Reichert Bright-Line Metallized Hemacytometer from Hausser Scientific (Horsham, PA).
The yeasts were centrifuged and washed twice in sterile saline, resuspended in saline, and enumerated with a hemocytometer.
Samples were enumerated with a hemocytometer for total leukocyte number.
Colonies were enumerated with a laser colony counter (Spiral System) after 48 h of incubation at 37°C.
Third, the S1-S2 components were enumerated with a 3′-dT20 appendage.
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