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The problems can be enumerated with a prim scowl and a wagging finger.
I sat with them on their mossy rear terrace while they enumerated, with a kind of mellow resignation, their house's problems.
The bacterial suspension in PBS was enumerated with a UV-visible spectrophotometer and inactivated with 1% (v/v) formalin for 1 h at 30°C.
Bead numbers were enumerated with a Reichert Bright-Line Metallized Hemacytometer from Hausser Scientific (Horsham, PA).
The yeasts were centrifuged and washed twice in sterile saline, resuspended in saline, and enumerated with a hemocytometer.
Samples were enumerated with a hemocytometer for total leukocyte number.
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PBMC were enumerated with an automated Guava cell counter (Millipore, Billerica, MA).
The agar plates were incubated for 28 days at 37°C/5% CO2 and individual M. avium colonies were enumerated with an inverted microscope after 14 and 28 days of incubation.
Spots were enumerated with an AID ViruSpot plate reader and ViruSpot 3.3 software (Autoimmun Diagnostika GmbH, Germany).
After enumerating with a blood cell counter, the bacterial cells were washed three times with sterile PBS, re-suspended at a density of 1.33×108 cells/ml, and used for the bacterial challenge experiments.
The colonies grown in each plate were enumerated with the help of a colony counter as CFU.
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