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Upon decontamination the MS2 was collected from the filter samples and enumerated via plaque assay.
Satellite cells were enumerated via double labelling with an anti-Pax7 antibody and DAPI.
Both strains were grown overnight in Tryptic Soy Broth (TSB, Difco 211825) in a shaking incubator at 37°C, bacterial pellets frozen in 20% glycerol at −80°C, and enumerated via serial dilution on Tryptic Soy Agar (Difco 236950).
Similar(56)
Total mesophilic count (TMC) were determined on Plate Count Agar (PCA) (Oxoid), incubated aerobically at 30 ℃ for 72 h; presumptive S. maltophilia were enumerated on vancomycin imipenem amphotericin B (VIA) agar [35], incubated aerobically at 30 ℃ for 48 h.
Cells were microscopically enumerated and viability was assessed via Trypan Blue exclusion.
The tissue was processed using a sterile homogenizer, and serial 10-fold dilutions were plated via a spiral plater and enumerated as above.
A synthetic route enabling a library to be efficiently enumerated was optimised from the previous synthesis of 7 via intermediate 9.
Ms. Carpenter enumerated them.
He enumerated his process.
The uncountable dead were enumerated.
They enumerated rather than essentialized.
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