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To determine the effect of stable over-expression of individual E2F family members on asynchronous cell growth, we plated each E2F-transduced line at low density in high (10%) serum medium, and enumerated the cells at 24 hour intervals over a four day culture period.
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The number of cells spiked to each tube was unknown to the 6 different operators who then enumerated the cell numbers by CellSearch.
While the microcircuits within each MB compartment remain to be elucidated, our light level anatomical studies have enumerated the cell types present in each compartment.
In most cases, staining or impedance can be used to enumerate the cells of interest, but all the cells of interest must be isolated and concentrated for this to be of value.
In contrast, the CPK procedure does not involve labelling or enumerating the cells, but rather only uses the anti-epithelial cell adhesion molecule antibody coupled to iron particles to yield an enriched population of CTCs, which can be used for molecular analyses.
To enumerate the cell growth on the PU films and PU-MWNT composite films, cell proliferations were carried out using cellTiter 96 aqueous one solution assay after being cultured for 24, 48 and 72 h (Fig. 4p).
Template gates were used to enumerate the cell populations of interest in all of the assays.
The automatic cell counting software enumerated the captured cells in 3 s.
We enumerated the invading cells located outside the pericardium (dashed line in Figure 1D).
We first enumerated the number of cells on a bacterial lawn used in typical C. elegans experiments by counting colonies of serial dilutions of E. coli lawns washed off of standard 6 cm growth plates.
After 48 h, we harvested the cells, labeled them with a monoclonal CD138 antibody and enumerated the surviving CD138+ MM cells using single-platform flow cytometry, to assess the percentage of MM cell lysis in each sample relative to that obtained with the control antibody KLH, which induced negligible MM cell lysis (data not shown).
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