entry vector

USAGE SUMMARY

The phrase "entry vector" is correct and usable in written English.
It can be used in contexts related to computer science, mathematics, or data analysis, particularly when discussing input data or parameters for algorithms or models. Example: "The algorithm requires an entry vector that represents the features of the data point being analyzed."

✓ Grammatically correct

Science

Human-verified examples from authoritative sources

Exact Expressions

60 human-written examples

Using the Gateway technology, each Entry vector can be recombined into multiple Destination vectors.

Plosone

The PCR product was cloned in the pDONR207™ gateway entry vector (Invitrogen™) via BP recombination reaction.

Plosone

The transformation is plated onto ampicillin plates, selecting against any unrecombined Entry vector.

Plosone

The resulting amplicon was purified and recombined into the Gateway entry vector pDONR/Zeo (Invitrogen).

Plosone

Resulting DNA fragments were recombined into the entry vector pDONR207 (Invitrogen).

Plosone

The 4164 bp PCR product was crossed into the GATEWAY entry vector pDONR/Zeo using the manufacturer's protocol, resulting in the entry vector pDONR-SHI, and subsequently used to cross into the modified GATEWAY expression vector pET-SHI.

Plosone

The expression vector has a kanamycin resistance gene, while the entry vector contains the beta-lactamase gene providing carbenicillin resistance.

Plosone

Human POT1 coding sequence was obtained from Invitrogen's Ultimate ORF Clones collection in Gateway-compatible entry vector pENTR221-hPOT1.

Plosone

The three PCR fragments were inserted into the entry vector pENTR-D-Topo (ceQORH, AtHMA1) and pDONR221 (AtAATpDONR221

Plosone

The amplified cDNA was first introduced into the Gateway entry vector, pDONR (Invitrogen) and the DNA sequence analyzed.

Plosone

Accordingly, a new pDONR201 entry vector (pDONR201-ptsO) bearing a longer version of the ptsO gene was constructed.

Plosone

Expert writing Tips

Best practice

When describing experiments, clearly specify the "entry vector" used, including its name (e.g., pDONR221) and any relevant modifications to ensure reproducibility.

Common error

Avoid using "entry vector" and "destination vector" interchangeably. An "entry vector" is used for initial cloning, while a destination vector receives the DNA insert from the entry vector in Gateway cloning.

Linguistic Context

The phrase "entry vector" functions as a noun phrase, specifically identifying a type of plasmid used in molecular biology. It is used to describe a vector that receives a DNA fragment during the initial steps of cloning, according to Ludwig's examples.

Expression frequency: Very common

Frequent in

Science

100%

Less common in

News & Media

0%

Formal & Business

0%

Encyclopedias

0%

Ludwig's WRAP-UP

The term "entry vector" is a noun phrase widely used within the scientific domain, specifically in molecular biology and genetic engineering. Ludwig confirms it's grammatically correct and frequently used. It denotes a specialized plasmid designed to receive DNA fragments in cloning processes. The term's primary function is to identify a tool used in experimental procedures, which carries a formal and scientific register. Common "entry vectors" include the pDONR and pENTR series, crucial in Gateway cloning methods. When writing, it's important to not confuse it with other vectors such as "destination vector" or "expression vector", each having distinct roles in cloning and expression workflows.

More alternative expressions

Phrases that express similar concepts, ordered by semantic similarity:

cloning vector

Focuses on the vector's function in replicating a specific DNA fragment.

expression vector

Highlights the vector's use in expressing a particular gene.

recombinant vector

Highlights that the vector contains recombinant DNA.

transfer vector

Emphasizes the vector's role in delivering genetic material.

viral vector

Specifies the vector is derived from a virus.

plasmid vector

Specifies the vector as a plasmid, a common type of circular DNA.

shuttle vector

Highlights the vector is used to move genetic material between different organisms.

gene carrier

A more general term indicating the vector's function in transporting genes.

genetic vehicle

A broader term for something that carries genetic material.

gene delivery system

A more general phrase for the technique.

FAQs

How is an "entry vector" used in cloning?

An "entry vector" is typically used to clone a gene or DNA fragment of interest. This fragment is then transferred to a "destination vector" for further applications, such as protein expression or gene editing.

What are common examples of "entry vectors"?

Common "entry vectors" include the pDONR series (e.g., pDONR221, pDONR/Zeo) from Invitrogen, as well as pENTR vectors. These vectors facilitate efficient cloning using Gateway recombination technology.

What is the difference between an "entry vector" and an expression vector?

An "entry vector" is designed for initial cloning and storage of a DNA fragment, while an "expression vector" is specifically designed to express the gene of interest, containing elements like promoters and terminators.

How does Gateway cloning utilize the "entry vector"?

In Gateway cloning, the gene of interest is first cloned into an "entry vector" flanked by attL sites. This "entry vector" then undergoes recombination with a "destination vector" containing attR sites, resulting in the transfer of the gene into the destination vector.