After JAG-GR was activated, coordination between cell volume and S phase entry was lost in meristem cells, as seen before for WT sepal primordia.
Cell surface-exposed PS is specifically detected by PE-Annexin V, and during the late stages of apoptosis or necrosis, cell membrane integrity is lost, allowing entry of the DNA-binding dye 7-AAD.
Second, we have assumed no re-entry (given that grandfathered status is lost on exit).
Some researchers have suggested that it is lost shortly after entry or during reverse transcription.
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