Exact(3)
We quickly applied a BSL-2 Ebola virus entry assay for a repurposing screen of 600 selected drugs.
We applied a previously developed Ebola virus-like particle (VLP) entry assay for a drug repurposing screen of Food and Drug Administration (FDA -approved drugs.
The identification of estrogen receptor modulators and microtubule inhibitors in our screen validates the BSL-2 Ebola virus entry assay for the identification of virus entry inhibitors.
Similar(57)
For entry assay, HSV-2 at a MOI of 0.004 pfu/cell was adsorbed for 2 hours at 4°C on prechilled confluent Vero cells.
For entry assay, 0.5 1×105 cells per well in 96-well-plate were spin-infected with different MOIs of virus for 1 h and incubated for another 2 h at 37°C to ensure complete virus entry.
Although more experiments will be needed to fully understand the possible use of any of these compounds against Ebola virus infection, here we describe a surrogate Ebola virus entry assay that can be used for HTS in a BSL-2 facility for the rapid screening of extensive compound collections.
All the screening data obtained in this Ebola VLP entry assay are deposited in the PubChem database for open access.
For entry assays, cells were infected for 20 min, washed, fixed in 4% paraformaldehyde for 15 min at room temperature, and stained as described below.
Thus, in a functional viral entry assay utilizing the full Env trimer and CD4 CCR5+ cells, NBD-556 acts as a surrogate for CD4 and promotes entry.
The PhenoSense Entry assay (MonoGram) has been developed to assess resistance to entry inhibitors.
For virus entry assays, VeroE6, 16HBE, BEAS-2B and A549 cells were infected with SARS-CoVpp.
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