Sentence examples for entry and expression from inspiring English sources
It involves the formation of a cationic lipid DNA complex that interacts efficiently with the cell surface, leading to the entry and expression of the exogenous DNA.
The attachment of the peptide consisted of 12 amino acids on the C-terminus of adenovirus fiber protein significantly improved entry and expression of a β-galactosidase transgene into neuroglial cells such as astrocytes, and Schwann cells.
Two key steps are involved in the cationic lipid mediated gene transfer process: (1) interaction between the cationic liposomes and DNA leading to the formation of a complex and (2) interaction between the complex and the target cells leading to DNA entry and expression of the transgene.
All entry and expression recombinant clones are stored as DNA and as bacterial glycerol stocks (15% v/v glycerol) of single colony-selected E. coli transformants.
In case of experiments to measure soluble G binding, the cut-off indicates fluorescent intensity indicative of binding; In the case of experiments to measure entry, the cut-off indicates fluorescent intensity associated with entry and expression of red fluorescent protein.
WT and mutant HSPB8-GFP fusion constructs were PCR amplified with proof reading enzymes and gateway entry and expression clones were created in the pLenti6-DEST vectors.
The software has been designed to automatically generate and maintain a standardized nomenclature during all steps of the cloning process, utilizing unique and consistent identifiers for ORFs, PCR reactions, colony-PCRs, entry- and expression clones (Fig. 7).
All the reactions were performed following the product protocol, and all the Entry clones and Expression clones have been sequenced.
Non-immune serum did not decrease the reporter gene expression, whereas pre-incubation with IVIG effectively neutralized viral entry and gene expression.
The relative virus gene expression, as measured by western blotting, was also similar under these knockdown conditions confirming that virus entry and gene expression were unaffected by the depletion of these Rabs.
With endogenous over-expression of miR-200b, the proliferation of IEC-6 cells increased significantly by increasing S-phase entry and promoting expression of the protein cyclin D1.
