Color images of each entire tissue section were acquired using NanoZoomer Digital Pathology System (Nanozoomer 2.0-HT slide scanner (Hamamatsu, Hamamatsu City, Japan).
Thanks to the high signal-to-noise ratio, we can visualize selected biomarkers at low magnification (20 × ) across entire tissue sections, and thus assess their spatial heterogeneity.
Safranin-O staining was homogeneously distributed throughout the entire tissue strand, and cells within tissue strands also displayed a characteristic cobblestone-like morphology.
Furthermore, we show that predominant fibril orientations in entire tissue units can be rapidly assessed from Raman parameter maps.
Finally, we outline how MRI can be used to monitor tissue structure, composition, and function to improve the entire tissue engineering process.
Müller glia are excellent targets for secreting neurotrophins as they span the entire tissue, ensheath all neuronal populations, are numerous, and persist through retinal degeneration.
The ability of visualizing RollFISH signals across the entire tissue section allowed us to assess the extent of spatial heterogeneity of HER2, ER, and Ki-67, in a way that would not be possible with smFISH.
It was constructed to assess the entire tissue volumes instead of solely a single cross-section.
Using the developed algorithm, entire tissue volumes could be assessed and gradients could be handled in an indirect manner.
At various time points, the light intensities of the entire tissue area and of several spots over selected vessels are measured.
In our protocol, biopsy was omitted in cartilaginous lesions without radiographic aggressive behavior, and definitive histologic diagnosis was made on the entire tissue obtained by curettage.
