The functional loss due to the spinal cord hemisection was maintained throughout the entire time of the study in both groups.
Cells carrying a Myc-tagged AKAP12β transgene showed robust expression of AKAP12β after 1 day of doxycycline treatment with levels AKAP12β persisting over the entire time course of study (Figure 5A).
Normal tissue sparing goals were applied over the entire time period of study.
Three independent biologic replicates of the entire time course study for E2-treated and time-matched AO-treated groups of animals were used to generate transcript profiling data and for subsequent statistical analysis.
The overall average of all participants' CMA provides the adherence value of the entire time period of the study [ 28] and evaluates the relationship of adherence and drug effect [ 27].
While ADR6, ADR11 and ADS11-2 are over expressed during the entire time study (set 12 of cluster analysis), ADR12-2 starts with very low levels of expression during stages 2 and 3 to then become up-regulated during stages 4 7 (set 10 of cluster analysis).
Hereby, two types of analyses were performed: a global one focusing on the AMI-CFRs of the entire time span of the study (the "period") and a temporal one (the "trend"), focusing on its per-semester evolution and intended to refine the initial, global assessment by taking temporal evolution into account.
Both genes show up regulation during the entire time study however, reduced levels of expression in one order of magnitude were observed after completion of the cotyledonary functional transition (see Figure 6).
In the absence of literature regarding important departures of the trend, we arbitrarily fixed these boundaries at - 5% and + 5%, allowing for a maximal increase of + 41% or for a maximal decrease of -30%, respectively, of the AMI-CFR during the entire time span of the study.
No temperature issues were reported over the entire time period of the study.
