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The assay would be useful as a prelude to sequencing entire or partial DNA genome regions of various OPVs, including natural or recombinant OPVs and potentially dangerous bioengineered OPVs designed to express foreign DNA or other viruses.
Cabrita Reis' precarious structures, which also include entire or partial buildings sited outdoors (he will be building one in Regent's Park, at next year's Frieze Art Fair), are in one sense uninhabitable, like Rachel Whiteread's casts, the late Absalon's one-man Habitations, Gregor Schneider's terrifying houses, Mario Merz's Igloos, the rooms and lives Perec described.
In addition, 7 chromosomal regions ranging from 57 bp to 849bp that encode entire or partial integrated transposases share between 99% and 100% identity with the two plasmid transposases (Figure 1).
In all the animals that we examined we noted no fragments of cephalopod flesh, gladii (entire or partial), sucker rings, hooks or eye lenses; beaks were found in varying stages of digestion and no fish or crustaceans remains were observed.
PCR amplification and cloning of cDNAs proceeded as follows: PCR oligonucleotides pairs were designed to amplify the entire (or partial) cDNAs without start and stop codons to optimize expression from pTrcHis2 (Invitrogen), and to fuse the C-termini to a c-myc epitope and 6X-His tags within the vector.
They likely represent entire or partial separations of the matrix compartment.
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For 44 DIPG patients the entire brain or partial brain specimen with detailed autopsy report was available for examination, allowing extensive histological review of tumour spread and statistical testing related to tumour dissemination.
Gain of chromosome 17 is one of the most frequent genetic abnormalities observed in NBTs, and may involve either the entire chromosome or partial gain of the distal segment 17q21-qter [ 17].
Promotors were predicted based on search of entire (Cons: BRE/TATA) or partial (TATA) motif consensus located 15 to 30 nt (otherwise pointed out in parenthesis) upstream of the 5' transcription start.
Loss of 9p, either through entire chromosome 9 loss or partial 9p loss alone, was observed in 15/20 cases (75%).
Gene conversion events involving Alu elements can be complete, whereby the entire element is converted, or partial, such that only a short stretch of sequence within the element is affected.
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