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Our assay allows quantification of additional aspects of diuresis, enteric function, and food intake.
In particular, Cognigni et al developed a method that allows quantification of diuresis, enteric function, and food intake, and showed complex dietary and reproductive modulation of some of these features.
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An important arbiter of these processes is the enteric nervous system (ENS), a network of neurons and glia within the wall of the bowel that controls most aspects of intestinal function.
Enteric neuronal function abnormalities are associated with a reduced amplitude of nerve inhibitor impulses on colon circular muscular layer and, hence, with a lack of coordination between colonic segments [ 32].
Furthermore, ROS appear to cause intestinal ileus via multiple mechanisms, which alter both muscle and enteric neural functions.
Inflamed GIT lead to persistent changes in enteric nervous system, the intrinsic innervation of the bowel which controls virtually all intestinal functions (motility, secretion, blood flow, mucosal growth and aspects of the local immune system), and smooth muscle function.
The data further suggest changes in enteric nervous system function and possibly brain development/function in the absence of NET function.
Taken together, over-expression of Cart, Htr3a and/or Tlx2, is likely to cause a perturbation in noradrenergic cell differentiation and in enteric nervous system function in NETKO mice.
The impact of bacteria on intestinal barrier function is clearly illustrated by the action of specific pathogenic enteric bacteria that have evolved remarkable means to penetrate and circumvent this important host defense mechanism.
Intestinal ischemia and reperfusion injury results in damage to elements critical to maintaining intestinal barrier function, including neurons and glia cells, which are part of the enteric nervous system (ENS).
The improvement in intestinal barrier function, therefore, likely prevented subsequent infiltration of immune cells and upregulation of cytokine expression in response to enteric antigens in the colon.
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