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We identify novel mechanisms ensuring specificity and robustness within stomatal Ca2+-signaling on a cellular, genetic, and biochemical level.
Other regions, including the ED (ERK docking) site, working with the CD domain and ensuring specificity and interaction strength [ 99].
Furthermore, PBD-phosphopeptide complex analysis revealed that an N-terminal Pro-residue plays a critical role in ensuring specificity for Trp414, Phe535, and Arg516 residues of PBD [ 80].
By crossing this line to Sox10 eGFP fish, we were able to photoconvert small numbers of neural crest cells, ensuring specificity of labeling.
Primers were designed such that the reverse primer was in the FLAG sequence (cIKK2) or myc sequence (DN-IκBα), ensuring specificity for transgene expression.
These findings provide genetic evidence for first genes that are essential for the ABA-triggered Ca2+ sensitivity priming in guard cells and show that these PP2Cs provide a mechanism ensuring specificity in Ca2+ signal transduction.
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These were included to ensure specificity in antigen antibody interactions in the experiments.
This approach ensures specificity and efficacy in fighting cancer cells, while healthy tissues remain largely unaffected.
Primers were designed for two variable regions of the NRPS gene sequence to ensure specificity for the emetic strains.
However, antibodies derived from in vitro selection or maturation lack an in vivo-like control system and rely on the counter screen to ensure specificity.
Amplification was performed in a thermal cycler (Appendorf) using a high stringency PCR protocol with high annealing temperature to ensure specificity of amplification.
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