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A threshold of 10-15 was used to ensure the stringency of locating matching genes.
For negative controls, ;;UAS-unfF1 virgins were routinely crossed to ;;;OK107-GAL4/+ or ;FRTG13UAS-mCD8/+;;OK107-GAL4/+ males at 22° to continuously monitor and ensure the stringency of the screen.
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We stress that respecting at least three stringency criteria will help to ensure the scientific quality of data interpretation of comparative studies: similar soil fertility status at start, comparable type of crop production, and quantification of off-farm organic and nutrient input.
The stringency of the threshold ensured that only sequences with extremely high selectively constraints are studied.
On the one hand the stringency of this approach ensures high specificity; on the other, misclassification error will tend to accumulate reducing the sensitivity of the overall DSM-IV algorithm.
Fourth, we have employed a high stringency selection in the YTH analysis by utilizing 4 different reporters to ensure the validity of the data.
While the stringency of mapping used here should ensure that we identify reads from the same population, it is possible that some mapped reads are from more distantly related members of the microbial community that are not part of the same population.
The stringency of this protocol is designed to ensure data quality, but carries the risk of sampling bias.
These problems might be alleviated by increasing the stringency of the proximity criteria on the colony picking device to ensure sufficient distance exists between two bacterial colonies before they are chosen for picking.
These inconclusive results could be due to the limited power and the stringency of our experiment.
Then they showed that the stringency of environmental regulation for manufacturing firms nearly doubled between 1990 and 2008.
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