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At least three biological replicates and three technical replicates were performed to ensure the quantification of each gene.
To ensure the quantification aspect of SeqZip, several conditions have to be examined: i) the specificity of the ligamers; ii) the equal hybridization and ligation efficiencies according to ligamer sequences; iii) the equal ligation efficiencies across various numbers of ligation events.
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It is the key to ensuring the quantification of time constraints.
To avoid sample loss during the desalting procedure, in-solution dimethyl labeling was performed to ensure the accuracy of the quantification.
The best-possible score (upper bound) of a metric can be obtained by ensuring the maximum quantification of the motif characteristics.
This step of the titration was repeated for each sample and it ensured the accurate quantification of newly formed FFA derived from the test samples.
In this work, we highlighted some potential disorders in relation to Bti spreading, and we are currently focusing our research on the improvement of the methodology to ensure the rapid and accurate quantification of bacterial DNA in leaf litter samples.
To ensure that quantification used an exposure that was within the linear range of the film used to image the blot, a series of images with increasing exposure time to the blot were developed.
To ensure the applicability of ATR FT-IR spectroscopy as a means for quantification of POX and PenV, spectra of synthetic single and binary solutions were acquired.
Careful validation of RGs has been repeatedly advocated as mandatory to ensure the accuracy of normalized data in qPCR studies adopting relative quantification.
To ensure the reliability of the data, values that were near or below the limits of the linear quantification were removed and excluded from further analyses.
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