Sentence examples for ensure the expression of from inspiring English sources

To ensure the expression of PbYOP1 in yeast, we utilized a 2μ vector with high copy numbers (pESC-URA) and drove protein expression using an inducible GAL promoter.

The primary aim of this study was to ensure the expression of CtxB antigen by the recombinant vaccine strain Z234-pMS101.

These compounds are either capable of correcting the trafficking of CFTR mutants ("correctors": agents that ensure the expression of the mutated protein at the apical plasma membrane) or improving channel function ("potentiators": agents that reinstate the channel function of mutated CFTR proteins that are orthotopically expressed).

After cultured for 24 h to ensure the expression of GFP-LC3, the cells were subjected to different treatment.

Media was replaced every 5 days and maintained for a minimum of 10 days before experimental use to ensure the expression of receptors.

To ensure the expression of target protein permanently, we chose the non-transcriptional spaces of yeast rRNA gene as chromosomal integration sites.

Generally, low temperature ensured the expression of less inclusion bodies and more soluble form of recombinant protein but some reports found that the soluble recombinant VEGF165 expression was increased when the cells were incubated at 37 °C (Lee et al. 2011).

The Sca1-based system ensures the expression of MafB into the HS/PCs compartment (Miles et al, 1997; Perez-Caro et al, 2009; Vicente-Duenas et al, 2012).

During autophagy, the cell conserves ATP by decreasing general protein synthesis in a linear fashion, proportional to the degree of flux change that is increased by autophagy, while ensuring the expression of proteins required for execution of autophagy as well as proteins that are critical in adaptation to starvation.

Bdnf is then produced, secreted and through its receptor initiates an autocrine loop that further inhibits MeCP2 function at two levels: (i) preventing MeCP2 DNA binding through phosphorylation mediated by CDKL5 and/or CAMKII and (ii) reducing MeCP2 levels by inducing miR-132 via CREB, thus ensuring the expression of Bdnf and other genes involved in neurite outhgrowth (Fig. 4).

The function of CTCFL might be to ensure the expression status of genes by distinguishing specific promoter-associated H3.3 domains from whole chromosome domains that also contain H3.3.