Sentence examples for ensure specificity of from inspiring English sources
To ensure specificity of signal, a specific phospho-blocking peptide for phospho Ser-396 was used on adjacent serial sections (BioSource), which completely blocked the signal from the primary antibody.
To ensure specificity of the primer to the specific homolog, the homologs were aligned using ClustalX [ 122] and primers were designed on divergent fragments of the sequence.
Amplification was performed in a thermal cycler (Appendorf) using a high stringency PCR protocol with high annealing temperature to ensure specificity of amplification.
To ensure specificity of OT effects to imitative behaviour, participants additionally performed a Stroop colour-word interference task (adapted to optimize similarities with the imitation inhibition task) to rule out general effects on cognitive control.
"There's a large amount of validation to ensure specificity of the antibodies and to make sure that the antibody is actually recognizing the intended target," says Craig M. Thompson, director of production and molecular assays, with Cell Signaling Technology (CST), located in Danvers, Massachusetts.
Staining was verified using the appropriate positive and negative controls to ensure specificity of staining.
The latter internal control ensured specificity of EdU detection to germ cells exposed to EdU.
By crossing this line to Sox10 eGFP fish, we were able to photoconvert small numbers of neural crest cells, ensuring specificity of labeling.
This strategy also ensured specificity of amplification, as the 3'-UTRs of most Hsp genes are divergent (even when CDSs are highly similar [ 40]).
This design ensures specificity of the singleplex PCR and reduces the risk of contamination of the multiplex PCR since only products from the singleplex reaction are amplified to high copy numbers [ 51].
Internal controls on each slide validated the analysis of BM-lymphocytes, with autologous PC providing the positive control and autologous M/PMNs providing negative control populations on the same slide and ensuring specificity of the testing.
