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CM were collected, centrifuged and filtered using a 0.2 μm filter to ensure removal of any dead cells.
Lipoaspirates were digested in collagenase then triple washed in 5% dextrose in lactated Ringers solution to ensure removal of all collagenase.
A complete dental prophylaxis, which included flossing to ensure removal of all plaque, was performed by an appropriately trained professional at the baseline visit or on a separate visit within 7 days of baseline.
The functional filters were positioned in the slow circuit to ensure removal of cellular debris, DNA and endotoxin, and particles >0.6 um prior to return to animal (supplementary Figure 1).
This was thoroughly washed with citric acid and water to ensure removal of unreacted monomers.
Chemical and microscopic checks were undertaken to ensure removal of all contaminants prior to testing and core analysis.
Similar(17)
The alkaline enzyme treatment ensures removal of wool thereby making the structure more open.
This additional stringency of collecting just the top 1% of CD4+CD25high cells as Tregs ensured removal of most of the activated CD25low T-cells.
Prior to experimentation, parasite cultures were synchronized to early ring stage using 5% sorbitol, the procedure preformed twice consecutively ensuring removal of all parasites not at the ring stage.
This process ensured removal of any traces of genomic DNA contamination.
In all experiments, a 2.5-min predrug perfusion of artificial plasma ensured removal of endogenous GCs from the brain vasculature.
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CEO of Professional Science Editing for Scientists @ prosciediting.com