The control of the terminal functional groups is particularly important to ensure the reactivity of macromonomers in the radical copolymerization; the degree of functionality was determined by both n.m.r. and u.v.-vis.
It is also advised to first let the oligonucleotide react with reducing agents such as dithio threitol [12, 26, 29, 31] or tris 2-carboxyethyl phosphine (tris 2-carboxyethyl phosphiney disulphide linkages and ensure full reacTCEPty.
Also, while activation is favored by organic solvents and acidic pH, 28 coupling requires aqueous buffer to achieve the template effect and neutral or basic pH to ensure nucleophilic reactivity at the primer terminus.
The present results disclosed a high prevalence of hormone receptor expression in the primary tumor, whose positivity was ensured by reactivity to least one of the two markers (ER and/or PR) (Table 3).
The objective is to ensure that the reactivity devices requirements of the natural uranium cycle during normal and accidental situations are maintained with minimal modifications to the reactor regulating system, while maximizing the fuel management advantages of advanced cycles.
A time bin scoring procedure was used to ensure that taste reactivity components of different relative frequency were balanced in their contributions to the final affective hedonic/aversive totals [74].
Given the denser architecture and reduced reaction volume compared to the 64-well card, tests were performed to ensure no cross reactivity or carryover of primers between wells.
All nuclear reactor designs provide a balance between their inherent nuclear characteristics and the engineered reactivity control features, to ensure that changes in reactivity under all operating conditions are maintained within a safe range.
To ensure no cross-reactivity between the primary antibodies and the unrelated second antibodies, sections were incubated with only one primary antiserum and followed by both secondary antisera.
Based on these features, antibody selection should ideally ensure minimal cross-reactivity with healthy tissues, sub-nanomolar affinity to the target antigen and a long pharmacokinetic half-life combined with minimal immunogenicity [ 79].
