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Gene-specific primers (Tables 1 and 2) were designed using the Primer Express 2 software (Applied BioSystems, Warrington, UK), optimised to ensure specificity and tested against either a bovine genomic DNA standard curve or human universal reference total RNA (Takara Bio Europe/Clontech, Saint-Germain-en-Laye, France) standard curve, to ensure optimal efficiency.
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Highly expressed genes have been reported to use a restricted set of codons to ensure optimal translational efficiency [ 52, 53].
The reference genes were methodologically analyzed by a 10-fold dilution series of cDNA from MCF-7 in water 1-1 100.0000) to ensure optimal amplification efficiency.
This study dealt with the issue of analytical specificity and sensitivity by meticulously testing qPCR reaction conditions for all assays (e.g., PCR annealing temperature) in order to ensure optimal PCR efficiency, while maintaining stringency.
These full-spectrum protections must be carefully designed by selecting UV filters with highly reliable stability to ensure optimal safety and efficiency during the entire period of sun exposure.
Validation was performed to ensure optimal cDNA amplification efficiency of the target and reference genes.
To ensure optimal DNA polymerization efficiency, amplicon length ranged between 69 and 113 bp.
The amplicon lengths ranged from 68 to 138 bp, to ensure optimal polymerization and efficiency.
Primers were designed as previously described to ensure optimal DNA polymerization efficiency and amplification specificity, as well as optimal amplicon length (100 180 bp; Table 4).
However, there is an allied period of changeover with implementation that requires careful consideration to ensure optimal performance and efficiency of work tasks.
To ensure optimal photon detection efficiency, this fiber is coupled to a collimator and the collimated light beam is focused by a lens (focal length~2 cm) to the active area (diameter~20 μm) of a SPC module (id100-20, id Quantique).
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CEO of Professional Science Editing for Scientists @ prosciediting.com