Sentence examples for ensure its specificity from inspiring English sources

After this initial validation step, each pair of primers was further tested in real-time PCR reactions to ensure its specificity as determined by a unimodal-melting curve.

We have extensively characterized this antibody through Western blotting, immunoprecipitation, immunohistochemistry and immunoflourescence using multiple cell model systems which express either no ERs, ERα alone, ERβ alone or a combination of ERα and ERβ, to ensure its specificity for the β isoform.

To ensure the specificity of MIDA for its intended target, most members of a template are required to be present.

As Wang continues to refine his method, he aims to increase its specificity and ensure his capture compounds bind to the exact same proteins they would inside a live cell.

The final concentration of 600/600 nM was selected to ensure efficiency and specificity for its target based on the dissociation curve that showed a single, sharp peak, indicating that the primers amplify one specific target.

The sequences of the primers used for the qPCR experiments are presented in Table 2. Melting curve analysis was used to ensure the specificity of each primer pair.

To ensure good specificity and reproducibility, lengthy and comprehensive sample preparation procedures are often required [16 18].

To ensure primer specificity, we performed the experiments when the melting curve showed a single peak.

Primers were designed from the 3′ region of the gene to ensure gene specificity.

Technical controls without primary antibody were performed for each marker to ensure staining specificity.

Extracted female DNA was also tested to ensure male specificity.