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This ratio is required to ensure expression of LRRK2 in GFP expressing cells, as LRRK2 is a large protein that often expresses poorly.
An additional level of control can be obtained by placing a Lox-STOP-Lox (LSL) cassette containing a transcriptional terminator between the promoter and the cDNA, which will ensure expression of the cDNA only in cells expressing functional Cre recombinase.
Moreover, proper function of the ER is required to ensure expression of immune relevant surface molecules and secretion of cytokines [71].
Once again, to ensure expression originated from a single allele we restricted our analysis to males.
Finally, we compared small RNA SOLiD sequencing results from multiple, independently-derived hESC lines to ensure expression across biologically variable samples.
On the following day, the valve sections were washed three times with 4 ml PBS to eliminate any residual gentamicin, with a final replacement by fresh ECM without antibiotics, but with synthetic cCF10 pheromone (1 ng/ml) to ensure expression of prgB by the bacteria during the assays.
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Different cell types pose different challenges, ranging from engineering immune acceptance to ensuring expression of sufficient levels of insulin and of insulin secretion in response to glucose.
The use of lentiviral-based vectors allows to precisely dose infection rates, ensuring expression of no more than one antibody per cell.
Control plasmid pEGFP-C1 ensuring expression of EGFP was purchased from Clontech.
Succeeding studies used a flower-specific FBP1 promoter in Kalanchoë, Campanula, and D. caryophyllus, which provided a better flower longevity without other developmental effects, since the promoter ensured expression solely in flowers.
Although genomic hypomethylation appeared throughout the genome, the loss of methylation at CpG rich gene promoters did not present diffuse pattern but appeared rather organized ensuring expression of genes crucial for survival.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com