Sentence examples for ensure attachment from inspiring English sources

Briefly, 5 × 104 iPSCs were plated into the wells of a Matrigel-coated XF24 cell culture microplate and incubated with 10 μmol/L Y-27632 for 24 h to ensure attachment.

To ensure attachment when plating cells, dishes were treated with fibronectin (10 µg/ml) for 30 minutes then rinsed with warm, sterile PBS before adding cells.

The cells were incubated on poly- L-lysinated coverslips overnight to ensure attachment.

Macroscopic photos were taken to demonstrate successful attachment and RAFT TEs were physically dragged to ensure attachment was secure.

Aliquots of the cells were then placed on Thermanox coverslips and incubated at 4° for 1 hr to ensure attachment, followed by fixing in 2% osmium tetroxide.

Worm immunofluorescence staining: adult C. elegans hermaphrodites were washed in PBS, and germlines were dissected on poly-L-lysine treated slides, covered with a coverslip to ensure attachment to slide, and snap-frozen on aluminum blocks on dry ice.

The final step in the electrode preparation was to introduce GOx to the sensing window, where 5 μl of 7 mg ml−1 GOx was added to the window and left overnight to ensure its attachment to the electrode surface.

However existing scaffolds are less than ideal for actual applications, not only as they lack mechanical strength due to pore size and have regular distribution, but also they do not ensure cell attachment, in-growth and organisation.

Checkpoint or stress induced phosphorylations of the Tub4 complex components may ensure microtubule attachment to the SPB, fine-tune microtubule dynamics or induce nucleation of microtubules.

We recommend using gelatin coated slides to ensure proper attachment of muscle sections during sequential staining.

Furthermore, we demonstrate that this activity of Aurora is required to ensure monopolar attachment at meiosis-I, and we expect that the same will hold true for metazoans.