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New protocols are available to enrich for low-abundance mRNAs, target specific genes, or select ribosomes to ensure analysis of only translated RNAs.
Processed datasets are tagged with processing date and annotation build versions to ensure analysis reproducibility.
Therefore, our cohort would have to recruit 600 participants to be able to ensure analysis of 480 patients.
The additional tests and procedures that we developed to ensure analysis of only those transfectants with the desired replacement are also described here.
To ensure analysis of identical genetic material by PFGE and MLVA, we used DNA from the same culture for both methods.
All mutagenized DNAs were subjected to multiple independent PCR amplifications to ensure analysis of different strands of DNA; subclones derived from independent PCR amplifications are distinguished by different letters of the alphabet.
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This strain ensured analysis of functional proteins, because the two transgenes rescued the double mutant to fertility.
Taking the foregoing into account, viral load was categorised in the present study according to percentile distribution, leaving 10 copies as cut-off point for ensuring analysis quality.
The re-equilibration part of the gradient program was extended to accommodate adequate stability prior to each injection, while ensuring analysis did not exceed 23 min per sample making it attractive for application in large-scale studies.
Enrichment analysis ensures that analysis of Argonaute-interacting RNAs is not biased by nonspecific interactions of Argonaute family proteins with the most abundant cellular RNAs.
They performed a logistic regression analysis, including adjustment to ensure FNN analysis was statistically reliable.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com