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Although motoneurons or glia could be enriched to perform the microarray analysis, we believe the data obtained from RNA isolated from whole flies is valuable for obtaining a global view of transcriptional changes when mutant SOD1 is expressed in a cell -specific manner.
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Other authors have also found that service learning seems to leave students with personal insight and understanding of vulnerable groups, and to enrich their ability to perform population-based care (Clayton and Dilley 2009).
With the increasing volume of publicly available unigene and cDNA sequences emerging from large-scale EST sequencing projects, the conventional need to generate enriched genomic libraries and to perform the necessary sequencing can now be largely bypassed [ 17].
A logical extension to the use of targeted capture to enrich diagnostic targets is to perform the analysis of whole exomes.
A total of 10 ng of Tr. anilis total RNA was used as template for Clontech's SMARTer Ultra Low RNA Kit for Illumina Sequencing to perform polyA enriched first strand cDNA synthesis and 12 cycles of polymerase chain reaction amplification according to manufacturer's instructions.
In order to prepare plant-produced HA for animal trials, ground plant extracts were clarified and diafiltered to enrich and concentrate HA and to perform a buffer exchange.
Again, some of these GO terms and some related GO terms were already found to be enriched when we performed genome wide analysis of HBS containing genes in the first section.
In contrast, the term "nervous system development" (P < 0.01), with 64 differentially-regulated genes, was found to be enriched only after performing the analysis on the 770 Refseq list, again, suggesting the presence of NR2E1 binding sites within the promoter regions of genes enriched for this term.
Thus, we examined their data set (GEO, GSE17215) and found that our EMT signature was also significantly enriched when performing GSEA (p = 0.000, FDR = 0.000, normalized enrichment score = 2.01).
Thrombin cleaved, untagged PfSENP1 was enriched by performing a subtractive Ni2+-NTA pull-down.
The annotation information was enriched by performing multisequence alignment using CLUSTALW [ 19].
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