Exact(18)
In prior work we showed that clof/9-cis RA did not influence palmitate toxicity in alpha-cell enriched preparations [7].
In the present study alpha-cell enriched preparations from TO1317- or vehicle-treated rats exhibited the same palmitate toxicity; this was also the case when TO1317 was added to the culture medium.
To date, the most highly enriched preparations contain a large molar excess of PrP per infectious unit [8], [38] and it remains unclear what proportion of PrP species are actually infectious [8].
Depletion and purity were assessed by flow cytometry and found consistently to exceed 95%and70%0% in CD4+CD25+ (containing also Treg) depleted and Treg enriched preparations, respectively.
Pools of RNA from highly enriched preparations of these cell types were subjected to expression profiling using massively parallel signature sequencing (MPSS) and four different genome wide microarray platforms.
For the studies on the p53-null mammary cancer model we used mammary epithelial enriched preparations as previously described [ 12], for the MMTV-erbB2 and C3(1)/SV40 T-antigen models we used total mammary gland preparations.
Similar(42)
DEseq [ 35] was used to normalize raw transcript counts and to compare libraries from PST infected tissue and enriched haustoria preparations in order to determine potential transcript enrichment in isolated haustoria compared to whole infected material (version 1.10.1).
For that purpose, it is essential to achieve highly enriched HPTEC preparations from kidney tissue.
To obtain highly enriched SENA preparations for transplantation, cultures were treated with 0.3 µg/ml collagenase XI (Sigma) at 37°C for 10 min, thus gently detaching entire SENAs comprising aggregates of radial glial cells and neurons from the monolayer of neural precursor cells surrounding the aggregates.
FMRP was only enriched in preparations of Drep-2GFP extracts.
Oligomer and fibril enriched Aβ1-42 preparations were generated as previously described [ 30].
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