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We continually exposed 3 week-old Mecp2−/y and Mecp2+/y mice to an enriched environment for 14 days.
Mice raised in an enriched environment for only the first three postnatal weeks acquired a spatial navigation task (MWM) at a rate similar to that of animals enriched their entire lives, and faster than those which had never been enriched.
The mice explored the enriched environment for 2 h in the dark.
Mice were either removed from the home cage for immediate fixation (no stim) or allowed to explore an enriched environment for 25 min prior to fixation (stim).
Rabbits were supplied food (alfalfa cubes) and water ad libitum while under quarantine in an enriched environment for at least 3 days before experimental use.
P14 and adult P60 wild-type and knockout mice were either removed from the home cage for immediate fixation by transcardial perfusion (unstimulated) or allowed to explore an enriched environment for 25 min prior to fixation (stimulated).
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Also, I saw studies where enriched environments for rats improved aspects of vision damaged after early deprivation.
Rats at an age of 26 to 29 months exposed to enriched environments for a few months regained nearly normal walking and sensorimotor behavior of the hindlimbs and the typical age-related enlargement of RFs of the hindpaw was almost eliminated.
Research into refinement is still on the sideline, but it has been shown already that enriched environments for laboratory animals improve the reliability of test results (Cao et al. 2010; Glaser and Kiecolt-Glaser 2005).
He postulated that individuals who begin life with the advantage of genes which increase their ability relative to the average may also be born into homes that provide them with more enriched environments, for instance being more committed to learning and teaching.
The agar plate was incubated at 35°C in a carbon dioxide-enriched environment for 18 24 hours, and the broth was incubated at 35°C overnight, subcultured onto layered horse blood agar and incubated as above.
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