A 10-fold dilution of the ligation reaction was used in a pre-amplification reaction with non-selective primers complementary to the adapter sequences to enrich the templates.
Figure 2 Pathway GeneSWAPPER (PGS) can be utilized not only to project a new species network but also to enrich the existing template network.
We introduce the single-strand amplification PCR (SSA-PCR) technique where a lone known locus-specific primer is firstly used to enrich the targeted template DNA strand resulting in significant PCR product specificity during the second round conventional nested PCR.
Fifteen rounds of PCR amplification were performed to enrich the purified cDNA template.
Fifteen rounds of PCR amplification were carried out to enrich the purified cDNA template using PCR primer PE 1.0 and 2.0 (Illumina) with phusion DNA polymerase.
Fifteen rounds of PCR amplification were performed to enrich the purified cDNA template using PCR Primer PE 1.0 and PCR Primer PE 2.0 (Illumina)] with Phusion DNA Polymerase.
Fifteen rounds of PCR amplification were performed to enrich the purified cDNA template using PCR Primer PE 1.0 and PE 2.0 (Illumina) with Phusion DNA Polymerase.
Fifteen cycles of PCR amplification were carried out to enrich the purified cDNA template using PCR primers PE 1.0 and 2.0 (Illumina) with phusion DNA polymerase.
Williams's latest score is one the most compelling in his forty-year "Star Wars" career: Rian Johnson's film complicates and enriches the familiar template, and Williams responds with intricate, ambiguous variations on his canon of themes.
A first amplification reaction (first PCR) served to enrich the proper 16S rDNA template, which was not achieved when using long primers as those used in the following steps.
