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CD45 immunomagnetic depletion was performed for the purpose of triple-staining experiments only to enrich the samples with CTCs.
To enrich the samples for tumour cells, tissue specimens were obtained from the central part of the tumour specimens.
PCR amplification with 15 cycles using Phusion polymerase was performed with primers complementary to the adapter sequences to enrich the samples for the desired fragments.
To enrich the samples in sRNAs, 3 volumes of polyethylene glycol (MW 8000) and NaCl to final concentrations of 5% and 500 mM respectively were added.
In order to enrich the samples for neoplastic cells, normal tissue was removed from the samples using macro-dissection based on the frozen section histology.
For chemical analysis, the stems had their bark removed and the differentiating xylem tissue was scrapped in order to enrich the samples with cells produced during the treatment.
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The area of tumour was manually micro-dissected to enrich the sample.
On the other hand, simulations at mixed/exchanged resolutions by parallel multi-scale approaches enrich the sampling of conformational space.
In order to enrich the sample, we therefore decided to include only patients with at least three chronic conditions.
Individuals with a Short Physical Performance Battery score 8 ≤7 were preferentially enrolled (45% of the sample) to enrich the sample with individuals at high risk for major mobility disability.
Second, the low rate of PTB in the Chinese population required us to retrospectively enrich the sample to increase the rate of PTB.
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