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We sequenced 1047 positive plasmids from eight microsatellite enrich libraries and confirmed 257 microsatellite loci from SSR enrich library (Table 2).
Based on the negative finding above, we hypothesized that selection under in vivo conditions is needed to enrich library clones that are able to bind cellular receptors in tumors, penetrate the tumor tissue and are internalized into tumor cells under physiological circulation conditions after intravenous administration.
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Third, one could use subtractive hybridization to remove known viral genomic sequences to further enrich libraries in novel genomes.
Of the 41 genomic microsatellite markers designed from (AG n enriched library, 24 were subsequently employed for characterization on 30 genotypes of Indian arid region.
Interestingly, the estimate from ER ChIP enriched library agreed with the aCGH reasonably well too (Pearson's r = 0.673, see Fig. 1b).
A modified protocol of Fischer and Bachmann [ 23] was used for constructing the SSR enriched library.
The SSR enriched library was constructed from the genotype TMV2 following by modified method of Fischer and Bachmann [ 23].
The microsatellite enriched library was constructed [ 48] for the identification of (GT/CA)n and (GA/CT n repeats.
Four thousand recombinant clones from the (GT/CA) and (GA/CT) microsatellite enriched library were screened which resulted in the identification of 387 clones that were sequenced.
The simple sequence repeat (SSR)–enriched library was sequenced on a Roche 454 platform using the GS FLX Titanium reagents at Ecogenics GmbH.
Seven hundred clones containing microsatellite sequences from a Tsp-AG/TC microsatellite enriched library were identified and one-hundred and forty-four primer pairs designed and synthesized.
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