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Leading-edge subsets were defined for all statistically enriched function gene sets (P<0.05).
No significantly enriched function was detected for predicted targets of NC-1321 or NC-265.
The most significantly enriched function in novel CNVRs was neuroactive ligand receptor interaction functions including glutamate signaling pathway.
We used FunSpec [ 64], a web-based cluster interpreter that identifies enriched function annotations across numerous knowledge sources.
The most significantly enriched function by target genes of core DE miRNAs was cellular growth and proliferation, followed by gene expression (Fig. 6a).
Similar to the case for the inhibitor pulse in the glucose consumption phase, GO term analysis identified oxidoreductase activity as a significantly enriched function among the induced genes.
To that end, we clustered our genes by similarity of expression, and within the resulting clusters employed GO term enrichment techniques to characterize enriched functions.
GO term enrichment analysis was used to identify significantly enriched functions among the genes affected in the different conditions.
A gene set enrichment analysis based on gene ontology reveals enriched functions in all three electric organs related to transmembrane transport, androgen binding, and signaling.
GO enrichment analysis of these clusters revealed significantly enriched functions within the FBW, FU, FI and MH-associated protein clusters (Additional file 3: Table S1).
Although some significantly enriched functions overlapped in the two traits, the overall picture suggested distinct functional enrichment profiles, supporting the gF– gC distinction at the genetic level.
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