GO enrichment analyses identifies biological functions enriched in each cluster (Additional file 1: Tables S12 S17).
Functional enrichment analyses comparing the categories of genes significantly enriched in the canonical binding motif with categories identified on the basis of ChIP/chip results from naive cells (see "Materials and Methods") revealed 14 biological processes that were enriched in both analyses at the p < 0.01 level.
Two enrichment analyses were performed on the genes of interest by assessing: (1) enriched Gene Ontology (GO) categories, and (2) enriched KEGG pathways.
Comparisons with the outcomes of patients from conventional headache units would definitely enrich our analyses, yet an in-patient treatment is usually not available in such facilities.
Moreover, the use of additional instruments, such as organizational commitment questionnaire, job satisfaction survey, workload scale, or interpersonal conflict at work scale to supplement the MBI will enrich future analyses and provide more alternative explanations for what is and is not found [ 33, 50].
In the present work, there were 18,712 UniGene transcripts from P. trifoliata libraries of the CitEST database and we decided to incorporate UniGene sequences from C. sinensis and C. reticulata to be represented in the array and consequently enrich the analyses.
The enriched GO analyses of all significant differentially expressed genes between any two stages were associated with 110 categories (p < 0.0002 and FDR < 0.01, Additional file 4).
Additionally, data on other health and environmental risk factors, such as prenatal exposures to nicotine or alcohol, gestational age at birth, childhood exposures to toxicants such as lead and PCBs, and better socioeconomic information, would have enriched these analyses.
Cluster, GO and KEGG analyses enrich the functions and pathways of the diff genes and proteins.
Accordingly, we plan to conduct several empirical studies to inform, complement, and enrich planned ethical analyses.
