When another disastrous outbreak occurred in 2002, the inspection service concluded that voluntary measures were not enough and more stringent regulations were needed.
Looking at the facts, it could be argued that these weapons don't constitute enough threat to warrant more stringent legislation.
But marine ecologist Enric Sala of Scripps Institution of Oceanography in La Jolla, California, and others warn that even targeted marine reserves likely aren't not enough to resuscitate dwindling populations without more stringent overall limitations on fishing.
First, obviously, make sure it's small enough to comply with even these new, more stringent size limits.
While the coverage levels were high enough to warrant raising the QV threshold to a more stringent level, the lower (default) value was chosen to minimize the false negatives.
If we choose a more stringent cutoff threshold, we will not have enough stress TFs (say five) for each stress to see whether there exists cross-talk among different stresses.
We expected that a less stringent cut-off (e.g., 40%) would allow too many negative sequences in the phyloPWM, and that a more stringent cut-off (e.g., 90%) would not introduce enough variability.
Repression of the GAL1 promoter by dextrose in pCCM1LC was enough to cause deficient growth in non-fermentable substrates [ 14], but a more stringent mitochondrial deprivation of Ccm1p was required to dissect events at the molecular level.
Although the protocol has some limitations, we expect to learn enough about the intervention, delivery and effects to support a larger trial with a more stringent design and enhanced statistical power.
Sequence databases have introduced more stringent requirements for submitters meaning that running an annotation pipeline alone is not enough to ensure acceptance of the genome annotation [ 9, 10].
