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The optimal parameters can be used for sequence identification (as demonstrated using the 14 query sequences) and for the recognition of new sequence types just by applying them to enlarged datasets.
We disregarded the 20,000 additional purebred individuals comprised in the enlarged datasets exclusively for the calculation of efficiencies, accuracies and numbers of discrepant individuals.
An R2 of 0.69 CA/CD may be potentially acceptable given the complexity of analysing highly variable historic samples, while the remaining correlations for the CA/CM and CD/CM ratios are mainly promising for future experiments with enlarged datasets.
We analyzed all local and enlarged datasets with both programs under the same settings as in simulations A and B and evaluated the proportions of true hybrids among classifications that were discrepant between programs or datasets.
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We used a substantially enlarged dataset from the UK by estimating the odds ratios associated with screening in overlapping narrow age bands.
We used real genotypes of purebred Microcebus griseorufus and M. murinus from Tsimelahy to simulate 100 local datasets with the same size and composition as in simulation A. We then enlarged each dataset by adding 100 purebred individuals of each species, which we simulated based on purebred genotypes from Hazofotsy and Ambatoabo.
In our research, these formerly used data sets are combined to enlarge the datasets and 3618 samples are got.
Modification of this value by enlarging training dataset did not change the positions of the curve but reduced its resolution due to lower number of test points.
In addition to further technical improvements in metabolite detection sensitivity, samples from urine and other tissues are needed to enlarge our dataset.
In some cases, it was not possible to enlarge the dataset due to lack of any outparalogs, so we had no choice but to make phylogeny with Amphimedon or Nematostella sequences as the root of the tree.
Nevertheless, it would be very useful to enlarge the dataset.
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