The enlarged clustered clathrin assemblies characteristic of essentially-munsicin-null HeLa cells appear to be linked to physiological compensation.
However, in cells lacking strumpellin the β2AR puncta appeared enlarged, clustered near the nucleus, and showed strong co-localisation with SNX1 (Fig. 8C, D).
Further, we allow slight deviations which enlarge cluster shell by equal amount of additional Ch atoms and reduce the number of Ch atoms between neighboring As cations, until this procedure is possible for chosen ChG composition.
Exact centrosome numbers in these enlarged centrosomal clusters were difficult to determine owing to tight centrosome clustering.
Consequently, in Abd-B Uab1, a mutant specific for the r isoform (m+r-) (Casanova et al., 1986), we obtained almost the same phenotype as in the Abd-B loss-of-function alleles: significantly enlarged NB3-3 clusters and ectopic NB6-4 neuronal subclones in PS14, as well as ectopic NB7-3 lineages (co-expressing Eg, En and Ey) in PS15 (Fig. 4B, Fig. 9A).
According to the experiment, with the processing data scale being enlarged, the clustering rate is in nearly linear growth, and the proposed parallel spectral clustering algorithm is suitable for large data mining.
DOI: http://dx.doi.org/10.7554/eLife.04137.004 Following RNAi, the phenotype typical of FCHO2-depleted HeLa cells is a reduced surface clathrin density and apparently enlarged or clustered clathrin-coated structures (Mulkearns and Cooper, 2012; Umasankar et al., 2012; but see Cocucci et al., 2012; Henne et al., 2010).
Ablation of Ctnnb1 in muscles (in ACTA1- Ctnnb1−/− mice) enlarges AChR clusters and increased the central band area where AChR clusters are distributed (Li et al., 2008).
Since other organisms, and thus other clusters, may exist nearby, these far-jumping organisms may venture so far from their parents' locations that they fall into the purview of other clusters, rather than either enlarging the cluster in which they originated, or nucleating a new cluster.
More importantly, during a Golgi-CAD trafficking assay, neither nocodazole nor EspG treatment blocked ER export, but instead arrested Golgi-CAD trafficking at the ERGIC, resulting in enlarged p58-positive clusters.
