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While LacZ-expressing HEK293T cells contained only small aggregates that were barely detectable, polyQ79 expression markedly enlarged aggregates (Fig 6J), validating our new approach for aggregate quantitation.
Additionally, ectopic expression of FXTAS repeats in a variety of cells results in the formation of enlarged aggregates, which are dynamic and increase over time (Fig. 2).
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Importantly, both metformin and AMPKαCA further enlarged these aggregates (Fig 6J), congruent with diminished HSP levels.
In both homozygous CbCln3Δex7/8 and CbCln6nclf cells aged at confluent density, the Lamp 1 immunostain also revealed enlarged or aggregated lysosomes, which were not often observed in the aged wild-type cells, consistent with lysosomal defects.
In such circumstances, the result is a shortfall in the supply of hydrolytic enzymes for useful purposes and, above all, inadequate autophagy with an accumulation of malfunctioning, enlarged mitochondria and aggregates of aberrant proteins (see " Lipofuscin and postmitotic ageing").
It has been found that the hydrodynamic diameters of the protein aggregates are enlarged and the electrokinetic potentials of them get positive with increasing the concentration of the surfactants.
ADAM: Enlarged prostate, enlarged prostate, enlarged prostate!
Open image in new window Fig. 1 100 times enlarged appearance of fine aggregate.
To investigate the relationship between 'lymphoid chemokines' CXCL13 and CCL21 and the developmental dynamics of organized lymphoid tissue in rheumatoid synovitis, by determining their local production sites in the context of progressively enlarging perivascular cell aggregates.
During the following days, the tumor cell aggregates grew and enlarged within the alginate beads.
The mitochondrial abnormalities obtained through loss of PINK1 function consist of mitochondrial aggregates, swollen or enlarged mitochondria and the presence of a tubular mitochondrial network within the cells.
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