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Second, it is possible E.coli protein(s) exist that contain binding surfaces(s) analogous to binding surface(s) on TNF-α and enhancing TNF-α binding site affinity also enhances affinity for analogous sites on E.coli protein(s).
These 2 mutations are important because D158N results in a potential glycosylation site and S227N enhances affinity for human receptors.
It is not surprising that MBP increases the solubility of the target protein, but how the MBP fusion tag enhances affinity for the membrane fraction is unclear.
A strong epistatic effect (G3xT4) is also present, which further enhances affinity for the GT combination by 0.8 kcal/mole beyond that expected from the main effects of these two nucleotides.
Therefore, the exchange of phenylalanine to another aromatic amino acid only slightly affects binding by reducing the affinity to phospholipids but not altering the affinity for galactolipids, whereas removal of the aromatic amino acid at this position significantly enhances affinity for galactolipids without significant alterations of the affinity for phospholipids.
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Lipases/acyltransferases are a special group of lipases with highly enhanced affinity for other nucleophiles than water compared to classical lipases.
Introduction of Glu217 into trypsin variants containing the SSFI sequence exhibited enhanced affinity for the factor Xa ligands (2) and (3).
We provide evidence that the dominant contribution to improved PR-104A activity was enhanced affinity for the prodrug over-competing intracellular substrates.
Both IC50 and δE2 values show C2 to have a significantly enhanced affinity for PLA2 as compared with C6, even though both are iso-lipophilic (Tables 1 and 2).
Does CBC have enhanced affinity for certain transcripts?
However, the TDO5 micelle conjugate displayed high affinity and selectivity for its target Ramos cells, as a result of densely packed aptamers that could enhance affinity for the target.
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CEO of Professional Science Editing for Scientists @ prosciediting.com