The signal was enhanced adding enhancement solution (Perkin Elmer, Turku, Finland).
Bound antibodies were detected by time-resolved fluorescence using europium-labelled anti-human antibody (PerkinElmer) and dissociation-enhanced lanthanide fluorescence immunoassay enhancement solution (PerkinElmer) followed by europium signal acquisition (330 nm excitation, 620 nm emission) on a plate reader.
To enhance fluorescence, 200 μL DELFIA Enhancement solution (PerkinElmer Finland Oy) was added per well and incubated for 10 min with shaking.
The serum was diluted in PBS and analyzed in duplicate, and then biotin-conjugated goat anti-mouse heavy- and light-chain antibodies were added, followed by europium-labeled streptavidin (Delfia, Wallac OY, Turku, Finland), and enhancement solution (Delfia Wallac); the amount of antibody was detected by dissociation-enhanced time-resolved fluoroimmunoassays research fluorometer.
Plates were washed four times between each step and finally incubated with enhancement solution before measuring the fluorescence.
Therefore, the proposed method provides a useful lightness-contrast enhancement solution for the applications of image/video processing because of the flexible adjustability with image color preserving.
Inspired by the Multiscale Retinex model, S. Chen et al. propose a color enhancement solution that works in a sequential way.
Europium-labelled streptavidin and enhancement solution Wallac, Oy were purchased through Perkin Elmer, Australia.
The plates were washed, europium labelled streptavidin added, and then developed with enhancement solution.
Therefore, initial plating out of sera was carried out with the plate on ice, while enhancement solution was stabilised at 22°C prior to its addition.
After incubation at room temperature for 1 hour with slow shaking, the plates were washed and DELFIA® Enhancement solution (Perkin Elmer) (100 µl/well) added.
