Sentence examples for enhancement buffer from inspiring English sources
We have tried a variety of methods to overcome this effect, including centrifugation, filtration, temperature variation of sera and the enhancement buffer, as well as alternate buffers but, to date, nothing has eliminated the appearance of hot wells.
Washing was found to be a crucial factor in the maintenance of low background levels, all washing steps consisted of 5 aspiration/wash cycles except for the final wash prior to the addition of the enhancement buffer which consisted of 8 aspiration/wash cycles.
After another incubation and wash, enhancement buffer was added and the bound europium was measured by time-resolved fluorometry.
After the addition of the analyte (appropriate cellular lysate), the detection of signals relies on a lanthanide (Europium -conjugated antibody that is ablEuropium -conjugatedescent signantibodyenhancementhatth acisic enhablementobuffer.
For detection the plates were incubated with europium-avidin followed by enhancement buffer according to the manufacturers' instructions and fluorescent intensity measured using a fluorometer (Wallac Oy EG & G, PerkinElmer, Waltham, Masschusetts, USA).
Ten microliters per sample of each of the capture beads (Th1/Th2) were mixed with serum enhancement buffer and the mixture was then incubated at room temperature in the dark for 30 min with serum before mixing with 50 μl of Human Th1/Th2-II PE detection reagent.
Receptor 1 showed immediate responses toward Zn2+ and Al3+ ions through selective fluorescence enhancement in buffer-acetonitrile and dimethylformamide (DMF) solution, respectively.
The thermophysical properties of the nanofluid might lead to heat transfer enhancement in the buffer layer and thus improve the thermal characteristics of the exchanger.
UV vis absorption and fluorescence spectroscopic studies further display a high selectivity towards Cys over GSH/Hcy and other amino acids and a high sensitivity towards Cys (0.088 270 μM) with 95-fold fluorescence enhancement in a HEPES buffer solution (10 mM, pH = 7.4) containing 20% MeCN at room temperature.
= competitive Besides its sensitivity and capacity for multiplexing, the DELFIA system has several additional advantages, including the commercial availability of all necessary protein labeling reagents and streptavidin lanthanide conjugates, microtitre plates, assay buffers, and enhancement solutions.
PAniTNC1-modified GCE immersed in 1.0 M H2SO4 at 25 ± 2 °C for 2 s shows a large enhancement in charge in O2-saturated buffer when compared to N2 and normal buffer, indicating that the nitrogen and oxygen buffer interferes with the nanocomposite layer, thereby increasing the charge.
