Sentence examples for enhanced transformation efficiency from inspiring English sources

Thomson et al. [ 20] pretreated Saccharomyces cerevisiae with LiAc and DTT and reported enhanced transformation efficiency.

Our results support the observations of Altpeter et al. (1996a), who reported an enhanced transformation efficiency by reducing the total time taken for production of transgenic plants.

Moreover, knockout of ARP6 led to disruption of microtubule integrity, higher uptake and degradation of virulence proteins, and increased DNA stability inside the cells, all of which resulted in enhanced transformation efficiency.

Up to now, the SAAT has been successfully employed to enhance transformation efficiency in several plant species, including kidney bean [ 20], radish [ 21], flax [ 22] and chickpea [ 23].

We provide a stable S. meliloti hsdR deletion mutant showing enhanced transformation with Escherichia coli-derived plasmid DNA and demonstrate that using an E. coli plasmid donor, expressing S. meliloti methyl transferase genes, is an alternative strategy of increasing the transformation efficiency of S. meliloti.

With a lactose inducible promoter for CRISPR array expression, we significantly decreased the toxicity of CRISPR-Cas and enhanced the transformation efficiency, and successfully deleted spo0A with an editing efficiency of 100%.

Application of vacuum during infection and dehydration at co-cultivation also enhanced the transformation efficiency, as did resting after infection and before culturing onto the selection medium [ 50].

Because of low transferring efficiency of a bare plasmid, several physical (electroporation, ultrasound, gas-filled micro-bubbles) and chemical (liposomes) approaches have been exploited to enhance their transformation efficiency [17].

Our findings have identified molecular and cellular mechanisms regulating AMT and a potential target for enhancing the transformation efficiency in yeast cells.

To our knowledge, this is the first time large-scale identification of optimal codons in Citrus species and P. trifoliata, which could serve as a model repertoire to enhance the transformation efficiency.

Important research directions, entailing the hydrogen economy, process intensification, enhanced efficiency, transformation of underutilized energy resources (e.g., natural gas from remote and offshore locations), and biomass conversion with novel processing schemes, are outlined.