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However, the cost increases associated with enhanced sequence coverage may not justify a relatively moderate increase in polymorphic SNPs.
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Aside from aiming for increased coverage through the additional prefractionation of membrane proteins, our results indicate that improving protein and/or peptide separation leads to significantly higher identification rates as well as enhanced average sequence coverage.
The average sequence coverage was 38.7-fold.
Approximately 22 peptides were sequenced for each protein hit resulting in minimum sequence coverage of 46%.
The data quality was observed following to these parameters; depth of coverage (DoC), allele coverage ratios (ACRs) and sequence coverage ratios (SCRs).
The sequence coverage was 69%.
Percentage of sequence coverage was indicated for each protein assignment.
Almost full sequence coverage was provided on selective exclusion monitoring.
The sequence coverage was ∼30% (data not shown).
This approach allows one to obtain robust sequence coverage.
Human transferrin was identified with the sequence coverage of 68%.
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