Exact(16)
Significant helical-phase-dependent enhanced repression remains even when HU is deleted (Figure 5B).
Furthermore, the PPARδ agonist enhanced repression was more marked (Figure 4D, P<0.05).
Introduction of ALT-PTK6 resulted in enhanced repression of the β-catenin target genes compared with PTK6 YF alone.
In the special case of strong operator sites, i.e., K1, K2 <enhanced repression in Equation 1 can be simplified to R = 1+J/Pt.
Throughout our data analysis we adopt the definition of enhanced repression given in Equation (1), which is more appropriate for characterizing effects of DNA looping.
This enhanced repression is the ratio of measured reporter activities for a construct in which the auxiliary operator has been deleted to that for a construct containing both primary and auxiliary operators.
Similar(44)
In skin papillomas, deletion of HUWE1 stabilizes MIZ1 and enhances repression of Cdkn2b (p15INK4b) and Cdkn1a (p21CIP1) by the MYC/MIZ1 complex, correlating with enhanced tumorigenesis (Inoue et al, 2013).
In particular, our model explains the optimal O1 O3 separation of 92 bp observed in the wild-type operon, which coincides with a strong peak in the enhanced- repression curve (Figure 5A).
Furthermore, the high loop yield (0.929 0.992) confirms that enhanced gene repression is almost exclusively attributable to DNA looping.
Due to the tethering effect of DNA looping this concentration can be much higher than the bulk free LacR concentration [2], [7], leading to increased occupancy of the primary site by the repressor and enhanced gene repression.
The loop yield directly correlates with the J factor, operator occupancy, and enhanced gene repression as demonstrated by their identical dependence on DNA helical phase (Figures 5B, 6, and 7).
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