Sentence examples for enhanced recombineering methods from inspiring English sources

Using enhanced recombineering methods, we previously developed an "obligate" anaerobic Salmonella strain YB1 and ST4 with enhanced host safety and anti-tumor activity (Guo et al. 2015; Shi et al. 2016; Yu et al. 2012).

Details on recombineering methods have been presented elsewhere.

The editing deficient strain of E. coli, pheT(G318W), was constructed using established recombineering methods involving the lambda red/gam pKD46 plasmid (Datsenko and Wanner, 2000).

The recombineering methods outlined here and in our recent study provide a means to manipulate the Drosophila genome with base-pair precision.

Using a novel recombineering method, Oligo-Assisted AAV Genome Recombination (OAGR), this work demonstrates the ability to direct concatemerization specifically to a desired orientation in human cells.

A very efficient recombineering method is based on the Red recombination system of bacteriophage λ [ 6].

Such counter-selection strategies are, however, limited to fosmid or other low-copy-number vector targets because the relative inefficiency of the recombineering method makes it difficult otherwise to select negatively for desired recombinants.

A targeting vector was made using a two-step BAC recombineering method, as shown in Fig. 1A.

Seven strains, each with a single deletion spanning one of the rrn operons, were made using the recombineering method (Datsenko and Wanner 2000).

We generated a panel of genetically modified Ad5 vector genomes by rapid AdZ homologous recombineering method (Fig. 1A), based on red lambda genetics.

The targeting constructs (pBDT and pBDU) were generated by modifying the bacterial artificial chromosome (BAC) clone (RP23-324O18 derived from C57BL/6J) using recombineering method with the plasmids, pRedET (Gene Bridges GmbH, Heidelberg Germany) [ 31].