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Compared to hESC-CMs in age (2 weeks) and purity (48 65%) matched 2D monolayers, hESC-CMs in 3D patches exhibited significantly higher conduction velocities (CVs), longer sarcomeres (2.09 ± 0.02 vs. 1.77 ± 0.01 μm), and enhanced expression of genes involved in cardiac contractile function, including cTnT, αMHC, CASQ2 and SERCA2.
With longitudinal observation of IVIS monitoring, animals with FUS treatment showed significant promotion of LpDNA release into the CNS and demonstrated enhanced expression of genes upon sonication with FUS-BBB opening, while both the luciferase and GDNF protein expression were successfully measured via Western blotting.
Notably, Symbioflor 1 showed a more enhanced expression of genes encoding lipases compared to the pathogenic strains.
In addition to IFN-induced genes, NYVAC-C-ΔB19R induced the enhanced expression of genes involved in TOLL like receptor signaling and JAK/STAT pathways (figure S1A).
As expected, NYVAC-C-ΔB19R induced the enhanced expression of genes in the type-I IFN-induced gene pathways and IL-1R (inflammasome) in pDCs (figure S1A).
Downregulation of the inhibitory p50 homodimer apparently contributes to the enhanced expression of genes regulated through κB elements in the PFC in alcoholics.
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The sk156 phenotypes were dominant when crossed with WT, suggesting that the altered phenotypes in sk156 were tightly linked to enhanced expression of gene(s) adjacent to the T-DNA, rather than gene disruption.
Then, we studied the floral initiation marker gene AP1 and its regulator gene FD because enhanced expression of both genes well reflects the molecular basis of RfBP-induced early flowering [ 40].
Analysis of gene expression profiles revealed that Oct4-activator fusions specifically enhanced expression of many genes identified as required for pluripotency, but not of genes that drive differentiation.
Most associations were positive (120/146 genes), indicating that enhanced expression of these genes was associated with greater numbers of copy number transitions.
Further, although loss of function instability phenotypes have been demonstrated for many of the genes in model systems, we observed enhanced expression of most genes in tumors, indicating that over expression, rather than deficiency underlies instability.
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