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Four positive clones with enhanced copies of xylose reductase gene from the T. reesei Δxdh strain were obtained by transferring the pPtef1-ble-xyl cassette) into the T. reesei Δxdh protoplast.
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Expression and protein level of EPSPS gene was positively correlated with enhanced copy number [ 86].
Given that heightened REST function is not due to enhanced REST copy number or gene expression, we investigated possible post-transcriptional mechanisms of regulating REST function.
Adds the following updates and enhancements: -Enhanced copy feature added to browser and email (shift key + tap and drag to select text).
There has been increasing number of evidences showing that mutated genes with deleterious effects are purged from the genome through purifying selection whereas copies with enhanced functions are fixed in the population through positive or diversifying selection [ 28, 29].
The activation may also occur through an increased gene copy number and enhanced gene expression whereas the inactivation may also occur by decreasing the copy number and downregulation of gene expression.
DOI: http://dx.doi.org/10.7554/eLife.03498.003 To investigate how myosin VIII regulates cell plate positioning, we generated a construct encoding Myo8A fused to three tandem copies of monomeric enhanced GFP (hereafter referred to as Myo8A-GFP) and transformed Myo8A-GFP into the myosin VIII null plant.
Each pack contained a copy of the enhanced autopsy protocol, data collection proforma and sample containers, each labelled with a unique bar code.
Lack of one LGI1 copy confers an enhanced susceptibility to auditory-evoked seizures, as in some patients with ADLTE, while early onset spontaneous seizures occur in mice lacking two copies.
However, despite the use of strong promoters or strains containing the genes of interest in multiple copies, the desired strongly enhanced enzyme levels are often not obtained.
In each case, copy number was higher in the mutant, implying that overall mitochondrial DNA copy number increases with enhanced recombination.
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