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As shown in Figure 2E, myeloid cells expressing WHIM CXCR4 displayed a significantly (P<0.05) enhanced attachment to activated Huvec compared to WT control.
The results obtained with various isogenic strains made in various genetic backgrounds including an heterologous host (Lactococcus lactis) confirmed that PVL-positive strains have enhanced attachment to the ECM components, the actual target being heparan sulfates and not directly ECM proteins.
Enhanced attachment to Matrigel® or an increase in cellular proliferation could complicate the interpretation of invasion results.
The enhanced attachment to damaged airway epithelium could play a central role during bacterial colonization and the early stages of infection.
Clinical PVL+ S. aureus isolates have been reported to display an enhanced attachment to ECM proteins (type I collagen, type IV collagen, and laminin) that are abundant in the lung [5].
Again, the fact that Lactococcus lactis strain expressing LukS-PV signal peptide displays enhanced attachment to ECM proteins renders the "signaling hypothesis" less likely and argues for a direct local effect on the cell surface.
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A brief period of exposure to ambient air prior to immersion in fixative also enhanced attachment of the colonic tissue to the glass.
Tethered EGF had no effect on hepatocyte attachment, contrary to soluble hEGF, which slightly enhanced attachment on 500 μM synKRGD hydrogels compared to unexposed hydrogels, and which yielded a 1.8 fold increase on collagen I-coated TCP.
To emphasize this point, enhanced attachment by Agr mutants in static biofilm systems is not observed in flowing biofilm reactor systems [ 87, 90].
Specifically, we tested two predictions of this hypothesis: (i) Φ6 infection or attachment will result in enhanced attachment of subsequent viral particles and (ii) enhanced attachment will not occur in phage that utilize constitutively expressed lipopolysaccharide as a receptor.
The in vitro studies demonstrated that, compared with SW scaffolds, NF scaffolds enhanced attachment and proliferation as well as odontogenic differentiation of human DPSCs.
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