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Results and conclusions: the presence of protein in urine enhanced amplification while nitrites decreased amplification.
Although TBT-PAR enhanced amplification of recalcitrant DNA, PCR inhibition was probably not eliminated completely as evidenced by the varying yields.
Because the ASE serves as a depopulation mechanism in TPLIF, such enhanced amplification has direct implications to the quantification of signals typically measured in practice.
The enzymatically generated insoluble precipitate formed an insulting layer on the electrode surface and efficiently restricted the electron transfer of redox probe Fe CN 64−/3−, resulting in an enhanced amplification of impedimetric signal.
Here, we developed a colorimetric biosensing system for the detection of proto-oncogene K-ras based on enhanced amplification effect of DNA molecular machine, where dual isothermal circular strand-displacement amplification (D-SDA) occurs on two arms in one-to-one correspondence.
With h = 1, there was no enhanced amplification even for large values of k1.
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By itself, this mechanism only weakly amplifies signaling in a shallow PDGF gradient, but it synergizes with other feedback mechanisms to enhance amplification.
A genome walking procedure termed loop-linker PCR was developed by adding a loop-linker adapter to the unknown region digested with restriction enzymes, which enhances amplification efficiency by suppressing the generation of nonspecific PCR products (Trinh et al. 2012).
3% DMSO was included in all PCR reactions to enhance amplification.
Here we propose a new pair of primers designed to enhance amplification of the COI-5P region in a wide range of marine organisms.
For this reason, we lowered the T c to 79.4°C to further enhance amplification of minority mutant alleles and confirm the KRAS mutations we detected in the 4 discrepant samples.
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