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Increasing pH and IS enhanced aggregation, while increasing NOM decreased mean aggregate sizes.
We chose L3 larvae in order to minimize the effect of different turnover rates between aggregated and soluble proteins between lines with suppressed and enhanced aggregation, and boiling in SDS ensured complete dissolution of aggregates.
Shear stress also significantly enhanced aggregation of platelets that are exposed to supernatant from chlamydia-infected monocytes.
Systematic in vivo screens of Aβ(1 42) variants fused to GFP permitted the assignment of intrinsic aggregation propensities for natural amino acids, and indicated enhanced aggregation for amino side-chains with increasing hydrophobicity [66], [67].
When challenged with AS+ E. faecalis, 10 rabbits actively immunized against AS+ E. faecalis developed more significant vegetations than 9 animals immunized against AS− E. faecalis, and 9/10 succumbed compared to 2/9 (p<0.005), suggesting enhanced aggregation by IgG contributes significantly to disease.
While N-terminal Asp/Glu replacements suppressed aggregation, C-terminal substitutions enhanced aggregation.
However, other secondary pathways such as branching or heterogeneous nucleation from the fibril surface may also contribute to enhanced aggregation.
An increased Aβ42/Aβ40 ratio enhanced aggregation and neurotoxicity in vitro and caused memory deficits after a single intraventricular injection in wild-type mice [ 44].
In line with these arguments, a Ser129-to-alanine mutant of α-synuclein displays enhanced aggregation tendencies in vitro and in vivo.
Increasing amounts of Ficoll and dextran enhanced aggregation in a concentration dependent manner (4-fold and higher at 200 g/L).
Group 2 is noted to have enhanced aggregation activity of platelets, enhanced thrombin activity and acceleration of the thrombin formation and activation of II and III coagulation phases.
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